TRNzol Universal Reagent

Sabuwar dabarar haɓakawa don fa'idar samfuri mai faɗi.

Ana iya amfani da TRNzol Universal Reagent don ware RNA gaba ɗaya daga samfura kamar ƙwayoyin cuta, ƙwayoyin cuta, naman gwari, dabba, ƙwayar shuka da ruwan jiki tare da mafi kyawun ikon lysis da haɓaka ƙima. Yana kula da amincin RNA yayin da yake lalata sel da rushe abubuwan sel yayin samfurin homogenization. RNA da aka ware ta wannan samfurin na iya zama kai tsaye azaman samfura don ganowa ta ƙasa ko wasu aikace -aikace.

Cat. A'a	Girman shiryawa
4992730	100 ml

Aika mana imel

Bayanin samfur

Misalin Gwaji

Tambayoyi

Alamar samfur

Siffofin

Flexibility Babban sassauci: Yankin daji na fara samfurin ƙarar, ya dace da babban haɓakar samfurin ƙima a cikin amsa ɗaya.
Yield Babban yawan amfanin ƙasa: Hanyar hazo tana haɓaka yawan amfanin RNA a cikin samfurin.
Use Amfani mai yawa: Ya dace da samfura daban -daban kamar su tsirrai da nama, ƙwayoyin al'ada, jini, ruwan jiki, da sauransu.
■ Yin aiki da sauri: Ana iya samun DNA na kwayar halitta a cikin awa 1 ..

Musammantawa

Rubuta: Hazo bisa
Samfurin: Kwayar cuta, ƙwayoyin cuta, naman gwari, dabba, ƙwayar shuka, ƙwayoyin al'ada da ruwan jiki.
Manufar: RNA
Lokacin aiki: ~ Awa 1
Aikace -aikace: TRNzol Universal reagent yana rage gurɓataccen ƙazanta kamar DNA da sunadarai a cikin RNA da aka tsarkake, kuma ana iya amfani da shi kai tsaye don gwaje -gwajen ƙwayoyin halittu daban -daban kamar Northern Blot, Dot Blot, PolyA screening, in vitro translation, bincike na kariya na RNase, ginin cDNA. , RT-PCR, PCR na ainihi da jerin abubuwan da aka tsara.

Duk samfuran ana iya keɓance su don ODM/OEM. Don cikakkun bayanai,don Allah danna Sabis na Musamman (ODM/OEM)

Na baya: RNAprep Pure Micro Kit

Na gaba: RNAprep Kit ɗin Cell/Bacteria Kit

Workflow

Hanyar: 30 MG na hanta bera, 100 MG na ganyen shinkafa an tattara ta hanyar ruwa mai nika nitrogen; 1 × 10⁶Kwayoyin al'adun HepG2 da 700 μl Saccharomyces Cerevisiae matsakaici (OD600 = 0.9) an tattara su ta hanyar centrifugation. 1 ml na TRNzol Universal Reagent daga TIANGEN da samfuran da suka dace daga mai siyar da L da T an ƙara su zuwa kowane madaidaicin samfuri kuma an yi hakar RNA bayan ƙa'idodin da kowane mai siye ya bayar. Ƙarar elution ita ce 80 μl, 50 μl, 30 μl da 30 μl don samfuran huɗu bi da bi. 3 μl na sama ya ɗora a kan kowane layi.
MIII: TIANGEN Alamar III;
An gudanar da electrophoresis a 6 V/cm na mintina 30 akan agarose 1%.
Sakamako: TIANGEN TRNzol Universal Reagent na iya fitar da tsattsarkar tsarkin gaske da ingantaccen mutuncin RNA daga hanta bera, ganyen shinkafa, ƙwayoyin al'adu da samfuran yisti, tare da babban inganci. Ingancin RNA kwatankwacinsa ko kaɗan kaɗan sama da na samfuran L da T.

Tambaya: Toshewar shafi

A-1 Cell lysis ko homogenization bai isa ba

---- Rage amfani da samfur, ƙara yawan adadin lysis, ƙara homogenization da lokacin lysis.

A-2 Samfurin adadin ya yi yawa

---- Rage adadin samfurin da aka yi amfani da shi ko ƙara yawan adadin kuzarin lysis.

Tambaya: Ƙananan amfanin RNA

A-1 Rashin isasshen lysis na sel ko homogenization

---- Rage amfani da samfur, ƙara yawan adadin lysis, ƙara homogenization da lokacin lysis.

A-2 Samfurin adadin ya yi yawa

---- Don Allah koma zuwa matsakaicin ƙarfin sarrafawa.

A-3 RNA ba a fitar da shi gaba ɗaya daga shafi

---- Bayan ƙara ruwa RNase-Free, bar shi na 'yan mintoci kaɗan kafin a datse.

A-4 Ethanol a cikin mafi girma

---- Bayan rinsing, sake maimaita centrifuge kuma cire buhun wanki gwargwadon iko.

Ba a cire matsakaicin al'adar C-5 gaba ɗaya

---- Lokacin tattara sel, don Allah a tabbata an cire matsakaitan al'adu gwargwadon iko.

A-6 Kwayoyin da aka adana a cikin RNAstore ba su da tsattsauran ra'ayi

---- Yawan RNAstore ya fi matsakaicin matsakaicin al'adar salula; don haka yakamata a kara karfin centrifugal. An ba da shawarar yin centrifuge a 3000x g.

A-7 Ƙananan abun cikin RNA da yalwa a cikin samfurin

---- Yi amfani da samfuri mai kyau don sanin idan ƙarancin samfurin ya haifar da samfurin.

Tambaya: ƙasƙantar da RNA

A-1 Kayan ba sabo bane

---- Yakamata a adana sabbin kyallen takarda a cikin sinadarin nitrogen nan da nan ko kuma a saka su cikin reagent na RNAstore don tabbatar da tasirin hakar.

A-2 Samfurin adadin ya yi yawa

---- Rage adadin samfurin.

A-3 RNase gurbatawan

---- Ko da yake buffen da aka bayar a cikin kit ɗin bai ƙunshi RNase ba, yana da sauƙi a gurɓata RNase yayin aikin hakar kuma ya kamata a kula da shi sosai.

A-4 gurɓataccen Electrophoresis

---- Sauya mazubin electrophoresis kuma tabbatar da cewa abubuwan amfani da Load Buffer ba su da gurɓataccen RNase.

A-5 Ana yin lodin yawa don electrophoresis

---- Rage adadin lodin samfurin, lodin kowace rijiya kada ya wuce 2 μg.

Tambaya: Gurɓacewar DNA

A-1 Samfurin adadin ya yi yawa

---- Rage adadin samfurin.

A-2 Wasu samfurori suna da babban abun ciki na DNA kuma ana iya bi da su tare da DNase.

---- Yi maganin DNase na RNase-Free zuwa maganin RNA da aka samu, kuma ana iya amfani da RNA kai tsaye don gwaje-gwajen da suka biyo baya bayan magani, ko kuma za'a iya ƙara tsarkakewa ta kayan aikin tsarkakewa na RNA.

Tambaya: Yadda za a cire RNase daga kayan gwaji da gilashin gilashi?

Don gilashin gilashi, gasa a 150 ° C na tsawon awanni 4. Don kwantena na filastik, nutse cikin 0.5 M NaOH na mintuna 10, sannan a rinshe shi da ruwa mara RNase sannan a yi taɓo don cire RNase gaba ɗaya. Reagents ko mafita da aka yi amfani da su a cikin gwaji, musamman ruwa, dole ne su kasance marasa RNase. Yi amfani da ruwa mara RNase don duk shirye-shiryen reagent (ƙara ruwa zuwa kwalban gilashi mai tsabta, ƙara DEPC zuwa taro na ƙarshe na 0.1% (V/V), girgiza dare da autoclave).

Rubuta saƙonka a nan ka aika mana