RNAprep Kit ɗin Cell/Bacteria Kit
Siffofin
Ingantattun buffers da ladabi don ƙwayoyin al'ada da samfuran ƙwayoyin cuta suna yin tsari mai sauƙi da dacewa.
Que Unique DNase I yana rage gurɓacewar ƙwayoyin halittar DNA.
Musamman RNase-Free Filtration Columns CS yana kawar da wasu gurɓatattun abubuwa.
■ Babban tsarki da shirye-shiryen RNA ya dace da aikace-aikacen da ke ƙarƙashin ƙasa masu mahimmanci.
■ Babu hakar phenol/chloroform, babu LiCl da hazo ethanol, babu CsCl gradient centrifugation da ake buƙata, wanda ke sa tsarin ya zama amintacce kuma abin dogaro.
Aikace -aikace
■ RT-PCR.
B Northern Blot, Dot Blot.
Haɗin PCR na ainihi.
Analysis Chip analysis.
Ing Fitar da PolyA, fassarar in vitro, nazarin kariyar RNase da ƙyallen ƙwayoyin cuta.
Duk samfuran ana iya keɓance su don ODM/OEM. Don cikakkun bayanai,don Allah danna Sabis na Musamman (ODM/OEM)
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Abu: Kwayoyin Jurkat na Dan Adam (1 × 106 ) Hanyar: An ware RNA na Kwayoyin Jukat na Mutane ta amfani da RNAprep Pure Cell/Bacteria Kit. Sakamako: Da fatan za a duba hoton agarose gel electrophoresis na sama. 2-4 μl na 50 elul eluates an ɗora su akan kowane layi. An gudanar da electrophoresis a 6 V/cm na mintuna 30 akan 1% gel na agarose. |
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Abu: TOP10 E.coli (1 × 108) Hanyar: An ware RNA na TOP10 E.coli ta amfani da RNAprep Pure Cell/Bacteria Kit. Sakamako: Da fatan za a duba hoton agarose gel electrophoresis na sama. 2-4 μl na 50 elul eluates an ɗora su akan kowane layi. An gudanar da electrophoresis a 6 V/cm na mintuna 30 akan 1% gel na agarose. |
A-1 Cell lysis ko homogenization bai isa ba
---- Rage amfani da samfur, ƙara yawan adadin lysis, ƙara homogenization da lokacin lysis.
A-2 Samfurin adadin ya yi yawa
---- Rage adadin samfurin da aka yi amfani da shi ko ƙara yawan adadin kuzarin lysis.
A-1 Rashin isasshen lysis na sel ko homogenization
---- Rage amfani da samfur, ƙara yawan adadin lysis, ƙara homogenization da lokacin lysis.
A-2 Samfurin adadin ya yi yawa
---- Don Allah koma zuwa matsakaicin ƙarfin sarrafawa.
A-3 RNA ba a fitar da shi gaba ɗaya daga shafi
---- Bayan ƙara ruwa RNase-Free, bar shi na 'yan mintoci kaɗan kafin a datse.
A-4 Ethanol a cikin mafi girma
---- Bayan rinsing, sake maimaita centrifuge kuma cire buhun wanki gwargwadon iko.
Ba a cire matsakaicin al'adar C-5 gaba ɗaya
---- Lokacin tattara sel, don Allah a tabbata an cire matsakaitan al'adu gwargwadon iko.
A-6 Kwayoyin da aka adana a cikin RNAstore ba su da tsattsauran ra'ayi
---- Yawan RNAstore ya fi matsakaicin matsakaicin al'adar salula; don haka yakamata a kara karfin centrifugal. An ba da shawarar yin centrifuge a 3000x g.
A-7 Ƙananan abun cikin RNA da yalwa a cikin samfurin
---- Yi amfani da samfuri mai kyau don sanin idan ƙarancin samfurin ya haifar da samfurin.
A-1 Kayan ba sabo bane
---- Yakamata a adana sabbin kyallen takarda a cikin sinadarin nitrogen nan da nan ko kuma a saka su cikin reagent na RNAstore don tabbatar da tasirin hakar.
A-2 Samfurin adadin ya yi yawa
---- Rage adadin samfurin.
A-3 RNase gurbatawan
---- Ko da yake buffen da aka bayar a cikin kit ɗin bai ƙunshi RNase ba, yana da sauƙi a gurɓata RNase yayin aikin hakar kuma ya kamata a kula da shi sosai.
A-4 gurɓataccen Electrophoresis
---- Sauya mazubin electrophoresis kuma tabbatar da cewa abubuwan amfani da Load Buffer ba su da gurɓataccen RNase.
A-5 Ana yin lodin yawa don electrophoresis
---- Rage adadin lodin samfurin, lodin kowace rijiya kada ya wuce 2 μg.
A-1 Samfurin adadin ya yi yawa
---- Rage adadin samfurin.
A-2 Wasu samfurori suna da babban abun ciki na DNA kuma ana iya bi da su tare da DNase.
---- Yi maganin DNase na RNase-Free zuwa maganin RNA da aka samu, kuma ana iya amfani da RNA kai tsaye don gwaje-gwajen da suka biyo baya bayan magani, ko kuma za'a iya ƙara tsarkakewa ta kayan aikin tsarkakewa na RNA.
Don gilashin gilashi, gasa a 150 ° C na tsawon awanni 4. Don kwantena na filastik, nutse cikin 0.5 M NaOH na mintuna 10, sannan a rinshe shi da ruwa mara RNase sannan a yi taɓo don cire RNase gaba ɗaya. Reagents ko mafita da aka yi amfani da su a cikin gwaji, musamman ruwa, dole ne su kasance marasa RNase. Yi amfani da ruwa mara RNase don duk shirye-shiryen reagent (ƙara ruwa zuwa kwalban gilashi mai tsabta, ƙara DEPC zuwa taro na ƙarshe na 0.1% (V/V), girgiza dare da autoclave).
Kayan samfuran
ME YA SA ZABE MU
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