Kwayoyin TGuide/Kit ɗin Kwayoyin Halittar DNA

Cire DNA na kwayar halitta daga sel masu al'ada da kyallen dabbobi.

TGuide Cell/Tissue Genomic DNA Kit an tsara shi musamman don fitar da tsattsarkar DNA daga ƙwayoyin al'ada da kyallen dabbobi, kyallen da aka saka da paraffin, kumburin baki, da busasshen jini ta amfani da TGuide Series Automated Nucleic Acid Extractor. Kit ɗin ya ƙunshi reagents da abubuwan amfani da ake buƙata don haɓakar DNA ta atomatik ta hanyar hanyar bead magnetic. An saka kayan reagents a cikin kwandunan reagent da aka rufe. Keɓaɓɓun guntun beads na magnetic da cikakken tsarin hakar atomatik suna tabbatar da tsarkakewar DNA cikin sauri da dacewa.

Cat. A'a	Pgirma Size
OSR-M401	48 preps

Aika mana imel

Bayanin samfur

Misalin Gwaji

Tambayoyi

Alamar samfur

Aikace -aikace

Ana iya amfani da DNA ɗin da aka tsarkake kai tsaye a cikin PCR, PCR mai yawa, Haɗin Kudancin, ƙuntata enzyme da sauran gwaje -gwaje.

Siffofin

■ Sauƙaƙe da hakar sauri: samfuran kayan haɗi na TGuide sun dogara ne akan ƙa'idar tsarkakewar acid na nucleic ta beads magnetic, kuma ana iya kammala tsarin haɓakar DNA na genomic a cikin mintoci 33.
Results Sakamakon amintattu: DNA da aka fitar ta kit ɗin ba shi da ƙazanta kamar RNA da furotin, kuma ana iya amfani da shi kai tsaye don PCR ko ƙimar PCR mai ƙima.
■ Babu phenol da hakar chloroform: Babu wani sinadarin ƙwayoyin cuta da ke cutar da ɗan adam

Duk samfuran ana iya keɓance su don ODM/OEM. Don cikakkun bayanai,don Allah danna Sabis na Musamman (ODM/OEM)

Na baya: TGuide Plant Genomic DNA Kit

Na gaba: TGuide FFPE DNA Kit-Mataki Daya

TGuide Cells/Tissue Genomic DNA Kit ne ya fitar da Genomic DNA na sel guda uku na al'adu.
An narkar da DNA a cikin buhun allurar μl 100. Ƙarar girma: 10 μl.
1: COS7 (1.2 × 10⁷ sel)
2: Jurkat (1 × 10⁶ sel)
3: HEK293 (1 × 10⁶ sel)
Haɗin DNA na ƙwayoyin dabbobi daban -daban ta amfani da Kit ɗin TGuide/Tome Genome DNA Kit

Experimental Example

Tambaya: Ƙananan ko babu DNA a cikin mafi girma.

A-1 Ƙananan ƙwayoyin sel ko ƙwayoyin cuta a cikin samfurin farawa-Inganta yawan ƙwayoyin sel ko ƙwayoyin cuta.

A. An ba da shawarar a gauraya sosai ta hanyar bugun jini don sau 1-2. - Rashin isasshen ƙwayar sel wanda ke haifar da raguwar aiki na proteinase K. - Rashin isasshen ƙwayar sel ko lalacewar furotin saboda ƙarancin lokacin wanka mai ɗumi. Ana ba da shawarar a yanke nama a ƙananan ƙananan kuma ƙara lokacin wanka don cire duk abin da ke cikin lysate.

A-3 Rashin isasshen talla na DNA. —Ba ethanol ko ƙaramin kashi maimakon 100% ethanol da aka ƙara kafin a canza lysate zuwa ginshiƙi.

A. -Daidaita pH tsakanin 8.0-8.3.

Tambaya: DNA ba ya yin aiki da kyau a cikin gwaje -gwajen halayen enzymatic na ƙasa.

Ethanol mai raguwa a cikin mafi girma.

- Akwai rakodin wanki na PW a cikin sama. Ana iya cire ethanol ta hanyar karkatar da juzu'in juzu'i na mintuna 3-5, sannan a sanya shi a ɗaki mai ɗumi ko 50 ℃ incubator na 1-2 min.

Tambaya: Rushewar DNA

A-1 Samfurin ba sabo bane. - Cire samfurin DNA mai kyau azaman sarrafawa don sanin ko DNA a cikin samfurin ya ƙasƙantar.

A-2 Aiki kafin magani. -Yana haifar da yawan iskar nitrogen mai narkewa, dawo da danshi, ko yawan samfurin.

Tambaya: Yadda ake yin prereatment don hakar gDNA?

Dole ne shirye -shiryen shiryawa ya bambanta don samfurori daban -daban. Don samfuran shuka, tabbatar da niƙa sosai a cikin nitrogen mai ruwa. Don samfuran dabbobi, homogenate ko niƙa sosai a cikin nitrogen mai ruwa. Don samfuran da ke da bangon sel waɗanda ke da wuyar fashewa, kamar ƙwayoyin G+ da yisti, ana ba da shawarar yin amfani da lysozyme, lyticase ko hanyoyin inji don fasa katangar tantanin halitta.

Tambaya: Menene banbanci tsakanin kayan hakar gDNA guda uku 4992201/4992202, 4992724/4992725, 4992709/4992710?

4992201/4992202 Plant Genomic DNA Kit ya ɗauki hanyar tushen shafi wanda ke buƙatar chloroform don hakar. Ya dace musamman ga samfuran tsire -tsire iri -iri, kazalika da busasshen foda. Kit ɗin Tsarin Hi-DNAsecure shima tushen tushen shafi ne, amma ba tare da buƙatar hakar phenol/chloroform ba, yana mai da shi lafiya kuma ba mai guba ba. Ya dace da tsire -tsire masu yawan polysaccharides da abubuwan polyphenol. 4992709/4992710 DNAquick Plant System yana ɗaukar hanyar tushen ruwa. Ba'a buƙatar hakar Phenol/chloroform shima. Tsarin tsarkakewa yana da sauƙi da sauri ba tare da iyakance ga adadin farkon samfurin ba, don haka masu amfani za su iya daidaita adadin a hankali gwargwadon buƙatun gwaji. Ana iya samun babban gutsuttsuran gDNA tare da yawan amfanin ƙasa.

Menene ƙididdigar ƙimar gDNA daga samfurin jini na 1 ml ta Kit ɗin DNA na Jini na TIANamp?

An samo kwayar halittar DNA daga juzu'i daban -daban na samfuran jinin ɗan adam ta Kit ɗin DNA na Jini. Sakamakon shine kamar haka. An jera sakamakon a matsayin tunani kawai, ainihin sakamakon hakar ya dogara da yanayin samfura.

Tambaya: Za a iya amfani da 4992207/4992208 da 4992722/4992723 don fitar da ɗanyen jini na DNA?

Ana iya aiwatar da haɓakar DNA na ɗigon jini ta amfani da reagents da aka bayar a cikin waɗannan kayan guda biyu ta hanyar sauƙaƙe yarjejeniya zuwa takamaiman umarni don hakar DNA na ɗigon jini. Za'a iya bayar da kwafin taushi na ƙa'idar haɓakar DNA akan buƙata.

Tambaya: Lokacin neman TIANamp Genomic DNA Kit, yadda za a fasa sabbin kyallen a cikin dakatarwar sel?

Dakatar da sabon samfurin tare da 1 ml PBS, saline na al'ada ko buhun TE. Cikakken homogenize samfurin ta homogenizer kuma tattara precipitate zuwa kasan bututu ta centrifuging. Jefar da allahntaka, kuma sake dawo da hazo tare da 200 μl buffer GA. Ana iya yin tsarkakewar DNA na gaba bisa ga umarnin.

Tambaya: Yadda za a zaɓi samfurin don haɓakar DNA daga plasma, magani da samfuran ruwa?

Don tsabtace gDNA a cikin plasma, magani da samfuran ruwa, ana bada shawarar TIANamp Micro DNA Kit. Don tsabtace ƙwayar gDNA daga samfuran serum/plasma, ana bada shawarar TIANamp Virus DNA/RNA Kit. Don tsabtace gDNA na kwayan cuta daga samfuran jini da samfuran plasma, ana bada shawarar TIANamp Bacteria DNA Kit (yakamata a haɗa lysozyme don ƙwayar cuta mai kyau). Don samfuran yau, ana ba da shawarar Hi-Swab DNA Kit da TIANamp Bacteria DNA Kit.

Tambaya: Yadda ake zaɓar kayan don hakar gDNA daga samfuran fungi?

Kit ɗin Shuka na Tsaro na DNA ko Tsarin Tsirrai na DNAquick ana ba da shawarar don haɓakar ƙwayoyin halittar fungal. Don haɓakar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar cuta, ana ba da shawarar Kit ɗin DNA na Yisti TIANamp (lyticase yakamata a shirya shi da kansa).

Kit ɗin Haɗin Dna na Cell Free