TGuide Kit ɗin Kwayoyin Halittar DNA
Aikace -aikace
Ana iya amfani da tsararren ƙwayar halittar kai tsaye a cikin PCR, RT-PCR, rashi narkewar enzyme, Haɗin Kudancin da sauran gwaje-gwajen.
Siffofin
■ Sauƙaƙe da hakar mai sauri: samfuran kayan haɗi na TGuide sun dogara ne akan ƙa'idar tsarkakewar acid na nucleic by beads magnetic, kuma za a iya kammala hakar DNA ɗin a cikin mintoci 44.
Results Sakamakon amintattu: Halittar halittar DNA da kit ɗin ya fitar ba shi da ƙazanta kamar RNA da furotin, kuma ana iya amfani da shi kai tsaye don PCR ko PCR mai ƙima.
■ Babu phenol da haɓakar chloroform: Babu buƙatar ƙwayoyin ƙwayoyin cuta masu cutarwa ga jikin ɗan adam kamar phenol da chloroform.
Size Girman samfurin farko mai sassauƙa: 200 μl, 400 μl, 1.2 ml na jini gaba ɗaya ko 1-3 ml na leukocyte da aka riga aka ware za a iya fitar da su kai tsaye ta hanyar zaɓar kit ɗin da ya dace.
Duk samfuran ana iya keɓance su don ODM/OEM. Don cikakkun bayanai,don Allah danna Sabis na Musamman (ODM/OEM)
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Sakamakon kwayoyin halittar DNA da aka ciro daga 200 μl dukan jini da aka yi amfani da shi tare da maganin kashe kwayoyin cuta daban -daban |
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An narkar da DNA a cikin buɗaɗɗen ƙirar μl 200. M: 1 kb tsani 1-5 Gano masu hanawa daban-daban na maganin kashe kumburi. Gene gene: Cbl-b gene, 1.7 kb P: Kyakkyawan iko |
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Sakamakon kwayoyin halittar DNA da aka fitar daga jini 400 μl ko 600 μl |
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An narkar da DNA a cikin buɗewar allurar μl 100 Genomic DNA ya samo asali daga 400 μl ko 600 μl jini gaba ɗaya Saukewa: DL15000 Ƙarar girma: 2 μl |
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Sakamakon kwayoyin halittar DNA da aka fitar daga jini 1.2 ml |
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An narkar da DNA a cikin bukkar allurar μl 300 An fitar da DNA na halittar jini daga 1.2 ml na jini gaba ɗaya M: DL15000 Alamar; Ƙarar girma: 2 μl |
A-1 Ƙananan ƙwayoyin sel ko ƙwayoyin cuta a cikin samfurin farawa-Inganta yawan ƙwayoyin sel ko ƙwayoyin cuta.
A. An ba da shawarar a gauraya sosai ta hanyar bugun jini don sau 1-2. - Rashin isasshen ƙwayar sel wanda ke haifar da raguwar aiki na proteinase K. - Rashin isasshen ƙwayar sel ko lalacewar furotin saboda ƙarancin lokacin wanka mai ɗumi. Ana ba da shawarar a yanke nama a ƙananan ƙananan kuma ƙara lokacin wanka don cire duk abin da ke cikin lysate.
A-3 Rashin isasshen talla na DNA. —Ba ethanol ko ƙaramin kashi maimakon 100% ethanol da aka ƙara kafin a canza lysate zuwa ginshiƙi.
A. -Daidaita pH tsakanin 8.0-8.3.
Ethanol mai raguwa a cikin mafi girma.
- Akwai rakodin wanki na PW a cikin sama. Ana iya cire ethanol ta hanyar karkatar da juzu'in juzu'i na mintuna 3-5, sannan a sanya shi a ɗaki mai ɗumi ko 50 ℃ incubator na 1-2 min.
A-1 Samfurin ba sabo bane. - Cire samfurin DNA mai kyau azaman sarrafawa don sanin ko DNA a cikin samfurin ya ƙasƙantar.
A-2 Aiki kafin magani. -Yana haifar da yawan iskar nitrogen mai narkewa, dawo da danshi, ko yawan samfurin.
Dole ne shirye -shiryen shiryawa ya bambanta don samfurori daban -daban. Don samfuran shuka, tabbatar da niƙa sosai a cikin nitrogen mai ruwa. Don samfuran dabbobi, homogenate ko niƙa sosai a cikin nitrogen mai ruwa. Don samfuran da ke da bangon sel waɗanda ke da wuyar fashewa, kamar ƙwayoyin G+ da yisti, ana ba da shawarar yin amfani da lysozyme, lyticase ko hanyoyin inji don fasa katangar tantanin halitta.
4992201/4992202 Plant Genomic DNA Kit ya ɗauki hanyar tushen shafi wanda ke buƙatar chloroform don hakar. Ya dace musamman ga samfuran tsire -tsire iri -iri, kazalika da busasshen foda. Kit ɗin Tsarin Hi-DNAsecure shima tushen tushen shafi ne, amma ba tare da buƙatar hakar phenol/chloroform ba, yana mai da shi lafiya kuma ba mai guba ba. Ya dace da tsire -tsire masu yawan polysaccharides da abubuwan polyphenol. 4992709/4992710 DNAquick Plant System yana ɗaukar hanyar tushen ruwa. Ba'a buƙatar hakar Phenol/chloroform shima. Tsarin tsarkakewa yana da sauƙi da sauri ba tare da iyakance ga adadin farkon samfurin ba, don haka masu amfani za su iya daidaita adadin a hankali gwargwadon buƙatun gwaji. Ana iya samun babban gutsuttsuran gDNA tare da yawan amfanin ƙasa.
An samo kwayar halittar DNA daga juzu'i daban -daban na samfuran jinin ɗan adam ta Kit ɗin DNA na Jini. Sakamakon shine kamar haka. An jera sakamakon a matsayin tunani kawai, ainihin sakamakon hakar ya dogara da yanayin samfura.
Ana iya aiwatar da haɓakar DNA na ɗigon jini ta amfani da reagents da aka bayar a cikin waɗannan kayan guda biyu ta hanyar sauƙaƙe yarjejeniya zuwa takamaiman umarni don hakar DNA na ɗigon jini. Za'a iya bayar da kwafin taushi na ƙa'idar haɓakar DNA akan buƙata.
Dakatar da sabon samfurin tare da 1 ml PBS, saline na al'ada ko buhun TE. Cikakken homogenize samfurin ta homogenizer kuma tattara precipitate zuwa kasan bututu ta centrifuging. Jefar da allahntaka, kuma sake dawo da hazo tare da 200 μl buffer GA. Ana iya yin tsarkakewar DNA na gaba bisa ga umarnin.
Don tsabtace gDNA a cikin plasma, magani da samfuran ruwa, ana bada shawarar TIANamp Micro DNA Kit. Don tsabtace ƙwayar gDNA daga samfuran serum/plasma, ana bada shawarar TIANamp Virus DNA/RNA Kit. Don tsabtace gDNA na kwayan cuta daga samfuran jini da samfuran plasma, ana bada shawarar TIANamp Bacteria DNA Kit (yakamata a haɗa lysozyme don ƙwayar cuta mai kyau). Don samfuran yau, ana ba da shawarar Hi-Swab DNA Kit da TIANamp Bacteria DNA Kit.
Kit ɗin Shuka na Tsaro na DNA ko Tsarin Tsirrai na DNAquick ana ba da shawarar don haɓakar ƙwayoyin halittar fungal. Don haɓakar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar cuta, ana ba da shawarar Kit ɗin DNA na Yisti TIANamp (lyticase yakamata a shirya shi da kansa).
Kayan samfuran
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