RNAprep Pure Plant Plus Kit
Siffofin
■ Anyi niyya: An tsara shi musamman don samfuran tsire-tsire waɗanda ke da wahalar cirewa, kamar polysaccharides da tsire-tsire masu wadatar polyphenolics. An fi inganta tsarin, kuma sakamakon abin dogara ne.
Removal Ingantaccen cire gDNA: Ana samar da ingantaccen DNase I don saurin cire gDNA akan ginshiƙi.
■ Mai sauƙi da sauri: Ana iya kammala gwajin hakar RNA a cikin awa 1.
■ Amintacciya da ƙarancin guba: ba a buƙatar reagents na abubuwa masu guba kamar phenol da chloroform.
Aikace -aikace
Ana iya amfani da wannan kit ɗin kai tsaye don gwaje-gwajen ƙwayoyin ƙwayoyin cuta iri-iri kamar RT-PCR, PCR na ainihi, Northern Blot, Dot Blot, gwajin polyA, fassarar in vitro, nazarin kariyar RNase, da rufewa, da sauransu.
Duk samfuran ana iya keɓance su don ODM/OEM. Don cikakkun bayanai,don Allah danna Sabis na Musamman (ODM/OEM)
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An fitar da jimlar RNA daga ƙoshin ayaba, kankana, apple da pear, tubers na dankalin turawa mai daɗi da dankalin turawa, ganyen auduga, fure, alfalfa, shinkafa da allurar allurar pine bi da bi ta amfani da RNAprep Pure Plant Plus Kit. An ɗora 4-6 ofl na 30 elul eluates ta kowane layi. M: Alamar TIANGEN III; An gudanar da electrophoresis a 6 V/cm na mintina 30 akan agarose 1%. Sakamako: RNAprep Pure Plant Plus Kit na iya fitar da tsabtar tsarki, yawan amfanin ƙasa da ingantaccen mutuncin RNA daga samfuran tsiron polysaccharides & polyphenolics. |
A-1 Cell lysis ko homogenization bai isa ba
---- Rage amfani da samfur, ƙara yawan adadin lysis, ƙara homogenization da lokacin lysis.
A-2 Samfurin adadin ya yi yawa
---- Rage adadin samfurin da aka yi amfani da shi ko ƙara yawan adadin kuzarin lysis.
A-1 Rashin isasshen lysis na sel ko homogenization
---- Rage amfani da samfur, ƙara yawan adadin lysis, ƙara homogenization da lokacin lysis.
A-2 Samfurin adadin ya yi yawa
---- Don Allah koma zuwa matsakaicin ƙarfin sarrafawa.
A-3 RNA ba a fitar da shi gaba ɗaya daga shafi
---- Bayan ƙara ruwa RNase-Free, bar shi na 'yan mintoci kaɗan kafin a datse.
A-4 Ethanol a cikin mafi girma
---- Bayan rinsing, sake maimaita centrifuge kuma cire buhun wanki gwargwadon iko.
Ba a cire matsakaicin al'adar C-5 gaba ɗaya
---- Lokacin tattara sel, don Allah a tabbata an cire matsakaitan al'adu gwargwadon iko.
A-6 Kwayoyin da aka adana a cikin RNAstore ba su da tsattsauran ra'ayi
---- Yawan RNAstore ya fi matsakaicin matsakaicin al'adar salula; don haka yakamata a kara karfin centrifugal. An ba da shawarar yin centrifuge a 3000x g.
A-7 Ƙananan abun cikin RNA da yalwa a cikin samfurin
---- Yi amfani da samfuri mai kyau don sanin idan ƙarancin samfurin ya haifar da samfurin.
A-1 Kayan ba sabo bane
---- Yakamata a adana sabbin kyallen takarda a cikin sinadarin nitrogen nan da nan ko kuma a saka su cikin reagent na RNAstore don tabbatar da tasirin hakar.
A-2 Samfurin adadin ya yi yawa
---- Rage adadin samfurin.
A-3 RNase gurbatawan
---- Ko da yake buffen da aka bayar a cikin kit ɗin bai ƙunshi RNase ba, yana da sauƙi a gurɓata RNase yayin aikin hakar kuma ya kamata a kula da shi sosai.
A-4 gurɓataccen Electrophoresis
---- Sauya mazubin electrophoresis kuma tabbatar da cewa abubuwan amfani da Load Buffer ba su da gurɓataccen RNase.
A-5 Ana yin lodin yawa don electrophoresis
---- Rage adadin lodin samfurin, lodin kowace rijiya kada ya wuce 2 μg.
A-1 Samfurin adadin ya yi yawa
---- Rage adadin samfurin.
A-2 Wasu samfurori suna da babban abun ciki na DNA kuma ana iya bi da su tare da DNase.
---- Yi maganin DNase na RNase-Free zuwa maganin RNA da aka samu, kuma ana iya amfani da RNA kai tsaye don gwaje-gwajen da suka biyo baya bayan magani, ko kuma za'a iya ƙara tsarkakewa ta kayan aikin tsarkakewa na RNA.
Don gilashin gilashi, gasa a 150 ° C na tsawon awanni 4. Don kwantena na filastik, nutse cikin 0.5 M NaOH na mintuna 10, sannan a rinshe shi da ruwa mara RNase sannan a yi taɓo don cire RNase gaba ɗaya. Reagents ko mafita da aka yi amfani da su a cikin gwaji, musamman ruwa, dole ne su kasance marasa RNase. Yi amfani da ruwa mara RNase don duk shirye-shiryen reagent (ƙara ruwa zuwa kwalban gilashi mai tsabta, ƙara DEPC zuwa taro na ƙarshe na 0.1% (V/V), girgiza dare da autoclave).
Kayan samfuran
ME YA SA ZABE MU
Tun lokacin da aka kafa ta, masana'antar mu tana haɓaka samfuran ajin farko na duniya tare da bin ƙa'idar
na inganci na farko. Abubuwan samfuranmu sun sami kyakkyawan suna a cikin masana'antar kuma amintaccen aminci tsakanin sabbin da tsoffin abokan ciniki ..
- Tel: +86 010-59822688
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