Tsarin DNA na jini na RelaxGene (0.1-20ml)
Siffofin
Sample Samfurin sassauƙan samfurin farawa: 2-400 μg babban tsarkin DNA ana iya fitar da shi daga 0.1-20 ml na samfuran jini daban-daban.
■ Amintacce kuma abin dogaro: Babu gurɓataccen ƙwayoyin kaushi kamar phenol/chloroform.
Aikace -aikace
Ya dace da aikace-aikace daban-daban na yau da kullun, kamar ƙuntatawa narkewa, PCR, ginin ɗakin karatu, Kudancin blot (haɗin guntu na jerin abubuwan da aka tsara), da sauransu.
Duk samfuran ana iya keɓance su don ODM/OEM. Don cikakkun bayanai,don Allah danna Sabis na Musamman (ODM/OEM)
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Tsarkake DNA na ɗan adam daga 300 μl, 5 ml da 10 ml jinin anticoagulation na ɗan adam tare da EDTA ta amfani da Tsarin DNA na jini na RelaxGene. 3 μl na 200 μl, 500 μl da 1 ml eluates bi da bi an ɗora su akan kowane layi. M: TIANGEN Alamar D15000. Sakamakon gwaji: 0.1-20 ml na jini za a iya fitar da shi ta Tsarin Tsarin DNA na jini na RelaxGene tare da madaidaicin layi. |
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Kwatanta DNA na kwayar halittar da aka fitar daga 300 μl anticoagulation na ɗan adam tare da EDTA ta amfani da Tsarin DNA na jini na RelaxGene da makamantan samfuran daga wasu masu ba da kaya. M: TIANGEN Alamar D15000. An ɗora 3 ofl na 200 elul eluates ta kowane layi. Sakamakon gwaji: Kwatanta da sauran masu ba da kaya, RelaxGene Tsarin DNA na Jini na iya samun yawan amfanin ƙasa, tsarki da haɗin gDNA na jini. |
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An samo DNA na ƙwayoyin halitta daga samfuran jini ta amfani da Tsarin DNA na jini na RelaxGene. Samfurori: 300 μl jinin anticoagulation na ɗan adam tare da EDTA, EDTA/NaF, heparin da sodium citrate; gashi mai kumburi daga jini 2 ml; 10 μl jinin kaji da 200 μl jinin bera. An ɗora 3 ofl na 200 elul eluates ta kowane layi. M: Alamar TIANGEN D15000. Sakamakon gwaji: RelaxGene Tsarin DNA na jini za a iya amfani da shi sosai a cikin tsabtataccen DNA mai inganci na maganin kashe kashe ɗan adam tare da jiyya daban -daban gami da jini daga nau'ikan daban -daban. |
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Sauran Bayanan Reference |
A-1 Ƙananan ƙwayoyin sel ko ƙwayoyin cuta a cikin samfurin farawa-Inganta yawan ƙwayoyin sel ko ƙwayoyin cuta.
A. An ba da shawarar a gauraya sosai ta hanyar bugun jini don sau 1-2. - Rashin isasshen ƙwayar sel wanda ke haifar da raguwar aiki na proteinase K. - Rashin isasshen ƙwayar sel ko lalacewar furotin saboda ƙarancin lokacin wanka mai ɗumi. Ana ba da shawarar a yanke nama a ƙananan ƙananan kuma ƙara lokacin wanka don cire duk abin da ke cikin lysate.
A-3 Rashin isasshen talla na DNA. —Ba ethanol ko ƙaramin kashi maimakon 100% ethanol da aka ƙara kafin a canza lysate zuwa ginshiƙi.
A. -Daidaita pH tsakanin 8.0-8.3.
Ethanol mai raguwa a cikin mafi girma.
- Akwai rakodin wanki na PW a cikin sama. Ana iya cire ethanol ta hanyar karkatar da juzu'in juzu'i na mintuna 3-5, sannan a sanya shi a ɗaki mai ɗumi ko 50 ℃ incubator na 1-2 min.
A-1 Samfurin ba sabo bane. - Cire samfurin DNA mai kyau azaman sarrafawa don sanin ko DNA a cikin samfurin ya ƙasƙantar.
A-2 Aiki kafin magani. -Yana haifar da yawan iskar nitrogen mai narkewa, dawo da danshi, ko yawan samfurin.
Dole ne shirye -shiryen shiryawa ya bambanta don samfurori daban -daban. Don samfuran shuka, tabbatar da niƙa sosai a cikin nitrogen mai ruwa. Don samfuran dabbobi, homogenate ko niƙa sosai a cikin nitrogen mai ruwa. Don samfuran da ke da bangon sel waɗanda ke da wuyar fashewa, kamar ƙwayoyin G+ da yisti, ana ba da shawarar yin amfani da lysozyme, lyticase ko hanyoyin inji don fasa katangar tantanin halitta.
4992201/4992202 Plant Genomic DNA Kit ya ɗauki hanyar tushen shafi wanda ke buƙatar chloroform don hakar. Ya dace musamman ga samfuran tsire -tsire iri -iri, kazalika da busasshen foda. Kit ɗin Tsarin Hi-DNAsecure shima tushen tushen shafi ne, amma ba tare da buƙatar hakar phenol/chloroform ba, yana mai da shi lafiya kuma ba mai guba ba. Ya dace da tsire -tsire masu yawan polysaccharides da abubuwan polyphenol. 4992709/4992710 DNAquick Plant System yana ɗaukar hanyar tushen ruwa. Ba'a buƙatar hakar Phenol/chloroform shima. Tsarin tsarkakewa yana da sauƙi da sauri ba tare da iyakance ga adadin farkon samfurin ba, don haka masu amfani za su iya daidaita adadin a hankali gwargwadon buƙatun gwaji. Ana iya samun babban gutsuttsuran gDNA tare da yawan amfanin ƙasa.
An samo kwayar halittar DNA daga juzu'i daban -daban na samfuran jinin ɗan adam ta Kit ɗin DNA na Jini. Sakamakon shine kamar haka. An jera sakamakon a matsayin tunani kawai, ainihin sakamakon hakar ya dogara da yanayin samfura.
Ana iya aiwatar da haɓakar DNA na ɗigon jini ta amfani da reagents da aka bayar a cikin waɗannan kayan guda biyu ta hanyar sauƙaƙe yarjejeniya zuwa takamaiman umarni don hakar DNA na ɗigon jini. Za'a iya bayar da kwafin taushi na ƙa'idar haɓakar DNA akan buƙata.
Dakatar da sabon samfurin tare da 1 ml PBS, saline na al'ada ko buhun TE. Cikakken homogenize samfurin ta homogenizer kuma tattara precipitate zuwa kasan bututu ta centrifuging. Jefar da allahntaka, kuma sake dawo da hazo tare da 200 μl buffer GA. Ana iya yin tsarkakewar DNA na gaba bisa ga umarnin.
Don tsabtace gDNA a cikin plasma, magani da samfuran ruwa, ana bada shawarar TIANamp Micro DNA Kit. Don tsabtace ƙwayar gDNA daga samfuran serum/plasma, ana bada shawarar TIANamp Virus DNA/RNA Kit. Don tsabtace gDNA na kwayan cuta daga samfuran jini da samfuran plasma, ana bada shawarar TIANamp Bacteria DNA Kit (yakamata a haɗa lysozyme don ƙwayar cuta mai kyau). Don samfuran yau, ana ba da shawarar Hi-Swab DNA Kit da TIANamp Bacteria DNA Kit.
Kit ɗin Shuka na Tsaro na DNA ko Tsarin Tsirrai na DNAquick ana ba da shawarar don haɓakar ƙwayoyin halittar fungal. Don haɓakar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar cuta, ana ba da shawarar Kit ɗin DNA na Yisti TIANamp (lyticase yakamata a shirya shi da kansa).
Kayan samfuran
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