Tumbin Magnetic/Cell/Jinin Kit ɗin RNA
Siffofin
■ Mai sauƙi da sauri: Za a iya samun jimlar RNA a cikin mintuna 60.
■ Babban kayan aiki: Yana iya cika buƙatun hakar da hannu da kuma haɓakar batutuwa akan dandamali daban-daban.
■ Amintacce kuma mara guba: Ba a buƙatar reagent kamar phenol/chloroform.
Musammantawa
Nau'in: Haɗin nau'in beads na Magnetic.
Samfurin: Kwayoyi, sel da jini.
Manufa: Jimlar RNA
Fara farawa: 5-20 MG, kada ya wuce 1 × 107, 0.1-1.5 ml
Lokacin aiki: 60 min
Aikace-aikacen ƙasa: RT-PCR/RT-qPCR, ginin ɗakin karatu na NGS, da dai sauransu.
Duk samfuran ana iya keɓance su don ODM/OEM. Don cikakkun bayanai,don Allah danna Sabis na Musamman (ODM/OEM)
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An fitar da jimlar RNA daga hanta berayen 20 MG tare da TIANGEN Magnetic Tissue/Cell/Blood Total RNA Kit da samfuran da suka dace daga wasu masu siyarwa. An ɗora 5 μl na 200 μl eluate zuwa 1% electrophoresis na agarose, 6 V/cm. Kammalawa: Yawan hakar, tsarkin da kwanciyar hankali na TIANGEN Magnetic Tissue/Cell/Blood Total RNA Kit sun fi na sauran masu ba da kaya. |
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An fitar da jimlar RNA daga 20 MG na koda bera tare da TIANGEN Magnetic Tissue/Cell/Blood Total RNA Kit a cikin TIANGEN TGuide S32 ko Thermo Kingfisher Flex96 bi da bi. An ɗora 5 μl na 200 μl eluate zuwa 1% electrophoresis na agarose, 6 V/cm. Alamar: TIANGEN D15000 Alamar DNA. Kammalawa: Kayan Magnetic Tissue/Cell/Blood Total RNA Kit yana da kyakkyawan haɓakar hakar da tsarkin a cikin masu cirewa daban -daban. |
A-1 Cell lysis ko homogenization bai isa ba
---- Rage amfani da samfur, ƙara yawan adadin lysis, ƙara homogenization da lokacin lysis.
A-2 Samfurin adadin ya yi yawa
---- Rage adadin samfurin da aka yi amfani da shi ko ƙara yawan adadin kuzarin lysis.
A-1 Rashin isasshen lysis na sel ko homogenization
---- Rage amfani da samfur, ƙara yawan adadin lysis, ƙara homogenization da lokacin lysis.
A-2 Samfurin adadin ya yi yawa
---- Don Allah koma zuwa matsakaicin ƙarfin sarrafawa.
A-3 RNA ba a fitar da shi gaba ɗaya daga shafi
---- Bayan ƙara ruwa RNase-Free, bar shi na 'yan mintoci kaɗan kafin a datse.
A-4 Ethanol a cikin mafi girma
---- Bayan rinsing, sake maimaita centrifuge kuma cire buhun wanki gwargwadon iko.
Ba a cire matsakaicin al'adar C-5 gaba ɗaya
---- Lokacin tattara sel, don Allah a tabbata an cire matsakaitan al'adu gwargwadon iko.
A-6 Kwayoyin da aka adana a cikin RNAstore ba su da tsattsauran ra'ayi
---- Yawan RNAstore ya fi matsakaicin matsakaicin al'adar salula; don haka yakamata a kara karfin centrifugal. An ba da shawarar yin centrifuge a 3000x g.
A-7 Ƙananan abun cikin RNA da yalwa a cikin samfurin
---- Yi amfani da samfuri mai kyau don sanin idan ƙarancin samfurin ya haifar da samfurin.
A-1 Kayan ba sabo bane
---- Yakamata a adana sabbin kyallen takarda a cikin sinadarin nitrogen nan da nan ko kuma a saka su cikin reagent na RNAstore don tabbatar da tasirin hakar.
A-2 Samfurin adadin ya yi yawa
---- Rage adadin samfurin.
A-3 RNase gurbatawan
---- Ko da yake buffen da aka bayar a cikin kit ɗin bai ƙunshi RNase ba, yana da sauƙi a gurɓata RNase yayin aikin hakar kuma ya kamata a kula da shi sosai.
A-4 gurɓataccen Electrophoresis
---- Sauya mazubin electrophoresis kuma tabbatar da cewa abubuwan amfani da Load Buffer ba su da gurɓataccen RNase.
A-5 Ana yin lodin yawa don electrophoresis
---- Rage adadin lodin samfurin, lodin kowace rijiya kada ya wuce 2 μg.
A-1 Samfurin adadin ya yi yawa
---- Rage adadin samfurin.
A-2 Wasu samfurori suna da babban abun ciki na DNA kuma ana iya bi da su tare da DNase.
---- Yi maganin DNase na RNase-Free zuwa maganin RNA da aka samu, kuma ana iya amfani da RNA kai tsaye don gwaje-gwajen da suka biyo baya bayan magani, ko kuma za'a iya ƙara tsarkakewa ta kayan aikin tsarkakewa na RNA.
Don gilashin gilashi, gasa a 150 ° C na tsawon awanni 4. Don kwantena na filastik, nutse cikin 0.5 M NaOH na mintuna 10, sannan a rinshe shi da ruwa mara RNase sannan a yi taɓo don cire RNase gaba ɗaya. Reagents ko mafita da aka yi amfani da su a cikin gwaji, musamman ruwa, dole ne su kasance marasa RNase. Yi amfani da ruwa mara RNase don duk shirye-shiryen reagent (ƙara ruwa zuwa kwalban gilashi mai tsabta, ƙara DEPC zuwa taro na ƙarshe na 0.1% (V/V), girgiza dare da autoclave).
Kayan samfuran
ME YA SA ZABE MU
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