Magnetic Serum/ Plasma DNA Maxi Kit

Ya dace don fitar da DNA daga 2-5 ml na magani/plasma tare da babban kayan aiki.

Kit ɗin yana ɗaukar beads ɗin magnetic tare da aikin rabuwa na musamman da tsarin buffada na musamman don rarrabewa da tsarkake DNA mai inganci daga samfura kamar serum da plasma tare da ƙarar 2-5 ml. Ƙirƙiri na musamman na magnetic beads suna da alaƙa mai ƙarfi ga acid nucleic ƙarƙashin wasu yanayi. Lokacin da yanayi ya canza, beads ɗin magnetic suna sakin adon acid wanda ke haɗe da shi, don haka yana cimma manufar rabuwa da sauri da tsarkake acid. Dukan tsari yana da aminci da dacewa. Cikakken nucleic acid da aka fitar yana da yawan amfanin ƙasa, babban tsarki, tsayayye kuma ingantaccen abin dogaro, kuma ya dace musamman don hakar atomatik na manyan wuraren aiki.

Cat. A'a	Girman shiryawa
4992413	2 ml × 50 preps
4992414	2 ml × 200 preps
4992917	2 ml × 1000 preps

Aika mana imel

Bayanin samfur

Misalin Gwaji

Tambayoyi

Alamar samfur

Siffofin

Kit Kit ɗin zai iya cika buƙatun hakar da hannu tare da haɓakar batutuwa akan dandamali daban-daban.
Products Samfuran da aka samo ta kit ɗin sun cika buƙatun gwaje -gwajen gano ƙasa da nazarin NGS.
Product Wannan samfurin ya dace da samfuran magani da samfuran plasma tare da ƙarar 2-5 ml.

Duk samfuran ana iya keɓance su don ODM/OEM. Don cikakkun bayanai,don Allah danna Sabis na Musamman (ODM/OEM)

Na baya: Kit ɗin Magnetic Serum/Plasma DNA Kit

Na gaba: Ƙasa Magnetic da Kit ɗin DNA

cfDNA an ciro shi tare da TIANGEN Magnetic Serum/Plasma DNA Maxi Kit da samfur mai dacewa daga Mai ba da M daga samfuran serum 2 ml.

Hoto 1: Yi Agilent2100 bincike bayan ginin ɗakin karatu na cfDNA.

Tebur 1: Qubit HS: An gano maida hankali na DNA ta hanyar babban hankali Qubit.

Darajar Ct: sakamako mai yawa na qPCR tare da firam ɗin globulin.

Agilent 2100: Mayar da hankali a matsayi 300 bp na ginin ɗakin karatu na DNA wanda aka bincika tare da Agilent 2100 Chip.

Tambaya: Ƙananan ko babu DNA a cikin mafi girma.

A-1 Ƙananan ƙwayoyin sel ko ƙwayoyin cuta a cikin samfurin farawa-Inganta yawan ƙwayoyin sel ko ƙwayoyin cuta.

A. An ba da shawarar a gauraya sosai ta hanyar bugun jini don sau 1-2. - Rashin isasshen ƙwayar sel wanda ke haifar da raguwar aiki na proteinase K. - Rashin isasshen ƙwayar sel ko lalacewar furotin saboda ƙarancin lokacin wanka mai ɗumi. Ana ba da shawarar a yanke nama a ƙananan ƙananan kuma ƙara lokacin wanka don cire duk abin da ke cikin lysate.

A-3 Rashin isasshen talla na DNA. —Ba ethanol ko ƙaramin kashi maimakon 100% ethanol da aka ƙara kafin a canza lysate zuwa ginshiƙi.

A. -Daidaita pH tsakanin 8.0-8.3.

Tambaya: DNA ba ya yin aiki da kyau a cikin gwaje -gwajen halayen enzymatic na ƙasa.

Ethanol mai raguwa a cikin mafi girma.

- Akwai rakodin wanki na PW a cikin sama. Ana iya cire ethanol ta hanyar karkatar da juzu'in juzu'i na mintuna 3-5, sannan a sanya shi a ɗaki mai ɗumi ko 50 ℃ incubator na 1-2 min.

Tambaya: Rushewar DNA

A-1 Samfurin ba sabo bane. - Cire samfurin DNA mai kyau azaman sarrafawa don sanin ko DNA a cikin samfurin ya ƙasƙantar.

A-2 Aiki kafin magani. -Yana haifar da yawan iskar nitrogen mai narkewa, dawo da danshi, ko yawan samfurin.

Tambaya: Yadda ake yin prereatment don hakar gDNA?

Dole ne shirye -shiryen shiryawa ya bambanta don samfurori daban -daban. Don samfuran shuka, tabbatar da niƙa sosai a cikin nitrogen mai ruwa. Don samfuran dabbobi, homogenate ko niƙa sosai a cikin nitrogen mai ruwa. Don samfuran da ke da bangon sel waɗanda ke da wuyar fashewa, kamar ƙwayoyin G+ da yisti, ana ba da shawarar yin amfani da lysozyme, lyticase ko hanyoyin inji don fasa katangar tantanin halitta.

Tambaya: Menene banbanci tsakanin kayan hakar gDNA guda uku 4992201/4992202, 4992724/4992725, 4992709/4992710?

4992201/4992202 Plant Genomic DNA Kit ya ɗauki hanyar tushen shafi wanda ke buƙatar chloroform don hakar. Ya dace musamman ga samfuran tsire -tsire iri -iri, kazalika da busasshen foda. Kit ɗin Tsarin Hi-DNAsecure shima tushen tushen shafi ne, amma ba tare da buƙatar hakar phenol/chloroform ba, yana mai da shi lafiya kuma ba mai guba ba. Ya dace da tsire -tsire masu yawan polysaccharides da abubuwan polyphenol. 4992709/4992710 DNAquick Plant System yana ɗaukar hanyar tushen ruwa. Ba'a buƙatar hakar Phenol/chloroform shima. Tsarin tsarkakewa yana da sauƙi da sauri ba tare da iyakance ga adadin farkon samfurin ba, don haka masu amfani za su iya daidaita adadin a hankali gwargwadon buƙatun gwaji. Ana iya samun babban gutsuttsuran gDNA tare da yawan amfanin ƙasa.

Menene ƙididdigar ƙimar gDNA daga samfurin jini na 1 ml ta Kit ɗin DNA na Jini na TIANamp?

An samo kwayar halittar DNA daga juzu'i daban -daban na samfuran jinin ɗan adam ta Kit ɗin DNA na Jini. Sakamakon shine kamar haka. An jera sakamakon a matsayin tunani kawai, ainihin sakamakon hakar ya dogara da yanayin samfura.

Tambaya: Za a iya amfani da 4992207/4992208 da 4992722/4992723 don fitar da ɗanyen jini na DNA?

Ana iya aiwatar da haɓakar DNA na ɗigon jini ta amfani da reagents da aka bayar a cikin waɗannan kayan guda biyu ta hanyar sauƙaƙe yarjejeniya zuwa takamaiman umarni don hakar DNA na ɗigon jini. Za'a iya bayar da kwafin taushi na ƙa'idar haɓakar DNA akan buƙata.

Tambaya: Lokacin neman TIANamp Genomic DNA Kit, yadda za a fasa sabbin kyallen a cikin dakatarwar sel?

Dakatar da sabon samfurin tare da 1 ml PBS, saline na al'ada ko buhun TE. Cikakken homogenize samfurin ta homogenizer kuma tattara precipitate zuwa kasan bututu ta centrifuging. Jefar da allahntaka, kuma sake dawo da hazo tare da 200 μl buffer GA. Ana iya yin tsarkakewar DNA na gaba bisa ga umarnin.

Tambaya: Yadda za a zaɓi samfurin don haɓakar DNA daga plasma, magani da samfuran ruwa?

Don tsabtace gDNA a cikin plasma, magani da samfuran ruwa, ana bada shawarar TIANamp Micro DNA Kit. Don tsabtace ƙwayar gDNA daga samfuran serum/plasma, ana bada shawarar TIANamp Virus DNA/RNA Kit. Don tsabtace gDNA na kwayan cuta daga samfuran jini da samfuran plasma, ana bada shawarar TIANamp Bacteria DNA Kit (yakamata a haɗa lysozyme don ƙwayar cuta mai kyau). Don samfuran yau, ana ba da shawarar Hi-Swab DNA Kit da TIANamp Bacteria DNA Kit.

Tambaya: Yadda ake zaɓar kayan don hakar gDNA daga samfuran fungi?

Kit ɗin Shuka na Tsaro na DNA ko Tsarin Tsirrai na DNAquick ana ba da shawarar don haɓakar ƙwayoyin halittar fungal. Don haɓakar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar cuta, ana ba da shawarar Kit ɗin DNA na Yisti TIANamp (lyticase yakamata a shirya shi da kansa).

Kit ɗin Haɗin Dna na Cell Free