TIANquick FFPE DNA Kit

Tsabtace hanzari na DNA na awa ɗaya daga madaidaicin formalin, kyallen takarda da aka saka ba tare da maganin xylene ba.

Kit ɗin DNA na TIANquick FFPE yana ba da yanayin lysis na musamman da hanyar ɓarna ta musamman don cire DNA daga samfuran formalin-madaidaici da sakawa (FFPE). Wannan kit ɗin yana jujjuya haɗin giciye kuma yana rage raunin DNA wanda hanyar FFPE ta haifar. Hakanan yana haɗa takamaiman shafi na talla wanda gaba ɗaya ya guji yin amfani da abubuwa masu guba da haɗari masu haɗari kamar xylene da narkewar dare tare da proteinase K. DNA mai tsabta ya dace don ganewar asali da bincike na ilimi.

Cat. A'a	Girman shiryawa
4992296	50 prep

Aika mana imel

Bayanin samfur

Misalin Gwaji

Tambayoyi

Alamar samfur

Siffofin

Za'a iya gama dukkan aikin cikin sa'a guda.
X Babu xylene ko sauran sauran kaushi masu haɗari.
Could Za a iya ware DNA mai tsarkin tsabta ba tare da narkewar proteinase K cikin dare ba.

Aikace -aikace

PCR da RT-qPCR
Analysis Binciken jinsin SNP da STR
Research Binciken Pharmacogenomics

Duk samfuran ana iya keɓance su don ODM/OEM. Don cikakkun bayanai,don Allah danna Sabis na Musamman (ODM/OEM)

Na baya: Kit ɗin Halittar Jinin Halittar DNA

Na gaba: TIANamp FFPE DNA Kit

Halittar halittar DNA ta TIANquick FFPE DNA Kit da samfur mai dacewa daga Mai ba da A an haɓaka zuwa 190 bp, 300 bp, 750 bp guntu. Sakamakon ya nuna cewa ingancin haɓaka PCR na gDNA da TIANquick FFPE DNA Kit da Mai ba da A suka yi daidai.

Abubuwan farawa: Samfuran hanta linzamin FFPE (guda 5, 10 μm/yanki)
Tsarin: Bi umarnin kowane samfurin.
Gel loading:
gDNA ta 50 μl elution buffer ya ɓata shi kuma an ƙara shi zuwa 190 bp, 300 bp da 750 bp. An ɗora samfuran 5μl na samfuran PCR 20 perl a kowace layi akan 1% gel na agarose. An gudanar da electrophoresis a 6 V/cm na minti 20.
M: Alamar TIANGEN D2000;
T: Kit ɗin DNA na TIANquick FFPE;
A: Irin wannan samfurin daga Mai samar da A.

Tambaya: Ƙananan ko babu DNA a cikin mafi girma.

A-1 Ƙananan ƙwayoyin sel ko ƙwayoyin cuta a cikin samfurin farawa-Inganta yawan ƙwayoyin sel ko ƙwayoyin cuta.

A. An ba da shawarar a gauraya sosai ta hanyar bugun jini don sau 1-2. - Rashin isasshen ƙwayar sel wanda ke haifar da raguwar aiki na proteinase K. - Rashin isasshen ƙwayar sel ko lalacewar furotin saboda ƙarancin lokacin wanka mai ɗumi. Ana ba da shawarar a yanke nama a ƙananan ƙananan kuma ƙara lokacin wanka don cire duk abin da ke cikin lysate.

A-3 Rashin isasshen talla na DNA. —Ba ethanol ko ƙaramin kashi maimakon 100% ethanol da aka ƙara kafin a canza lysate zuwa ginshiƙi.

A. -Daidaita pH tsakanin 8.0-8.3.

Tambaya: DNA ba ya yin aiki da kyau a cikin gwaje -gwajen halayen enzymatic na ƙasa.

Ethanol mai raguwa a cikin mafi girma.

- Akwai rakodin wanki na PW a cikin sama. Ana iya cire ethanol ta hanyar karkatar da juzu'in juzu'i na mintuna 3-5, sannan a sanya shi a ɗaki mai ɗumi ko 50 ℃ incubator na 1-2 min.

Tambaya: Rushewar DNA

A-1 Samfurin ba sabo bane. - Cire samfurin DNA mai kyau azaman sarrafawa don sanin ko DNA a cikin samfurin ya ƙasƙantar.

A-2 Aiki kafin magani. -Yana haifar da yawan iskar nitrogen mai narkewa, dawo da danshi, ko yawan samfurin.

Tambaya: Yadda ake yin prereatment don hakar gDNA?

Dole ne shirye -shiryen shiryawa ya bambanta don samfurori daban -daban. Don samfuran shuka, tabbatar da niƙa sosai a cikin nitrogen mai ruwa. Don samfuran dabbobi, homogenate ko niƙa sosai a cikin nitrogen mai ruwa. Don samfuran da ke da bangon sel waɗanda ke da wuyar fashewa, kamar ƙwayoyin G+ da yisti, ana ba da shawarar yin amfani da lysozyme, lyticase ko hanyoyin inji don fasa katangar tantanin halitta.

Tambaya: Menene banbanci tsakanin kayan hakar gDNA guda uku 4992201/4992202, 4992724/4992725, 4992709/4992710?

4992201/4992202 Plant Genomic DNA Kit ya ɗauki hanyar tushen shafi wanda ke buƙatar chloroform don hakar. Ya dace musamman ga samfuran tsire -tsire iri -iri, kazalika da busasshen foda. Kit ɗin Tsarin Hi-DNAsecure shima tushen tushen shafi ne, amma ba tare da buƙatar hakar phenol/chloroform ba, yana mai da shi lafiya kuma ba mai guba ba. Ya dace da tsire -tsire masu yawan polysaccharides da abubuwan polyphenol. 4992709/4992710 DNAquick Plant System yana ɗaukar hanyar tushen ruwa. Ba'a buƙatar hakar Phenol/chloroform shima. Tsarin tsarkakewa yana da sauƙi da sauri ba tare da iyakance ga adadin farkon samfurin ba, don haka masu amfani za su iya daidaita adadin a hankali gwargwadon buƙatun gwaji. Ana iya samun babban gutsuttsuran gDNA tare da yawan amfanin ƙasa.

Menene ƙididdigar ƙimar gDNA daga samfurin jini na 1 ml ta Kit ɗin DNA na Jini na TIANamp?

An samo kwayar halittar DNA daga juzu'i daban -daban na samfuran jinin ɗan adam ta Kit ɗin DNA na Jini. Sakamakon shine kamar haka. An jera sakamakon a matsayin tunani kawai, ainihin sakamakon hakar ya dogara da yanayin samfura.

Tambaya: Za a iya amfani da 4992207/4992208 da 4992722/4992723 don fitar da ɗanyen jini na DNA?

Ana iya aiwatar da haɓakar DNA na ɗigon jini ta amfani da reagents da aka bayar a cikin waɗannan kayan guda biyu ta hanyar sauƙaƙe yarjejeniya zuwa takamaiman umarni don hakar DNA na ɗigon jini. Za'a iya bayar da kwafin taushi na ƙa'idar haɓakar DNA akan buƙata.

Tambaya: Lokacin neman TIANamp Genomic DNA Kit, yadda za a fasa sabbin kyallen a cikin dakatarwar sel?

Dakatar da sabon samfurin tare da 1 ml PBS, saline na al'ada ko buhun TE. Cikakken homogenize samfurin ta homogenizer kuma tattara precipitate zuwa kasan bututu ta centrifuging. Jefar da allahntaka, kuma sake dawo da hazo tare da 200 μl buffer GA. Ana iya yin tsarkakewar DNA na gaba bisa ga umarnin.

Tambaya: Yadda za a zaɓi samfurin don haɓakar DNA daga plasma, magani da samfuran ruwa?

Don tsabtace gDNA a cikin plasma, magani da samfuran ruwa, ana bada shawarar TIANamp Micro DNA Kit. Don tsabtace ƙwayar gDNA daga samfuran serum/plasma, ana bada shawarar TIANamp Virus DNA/RNA Kit. Don tsabtace gDNA na kwayan cuta daga samfuran jini da samfuran plasma, ana bada shawarar TIANamp Bacteria DNA Kit (yakamata a haɗa lysozyme don ƙwayar cuta mai kyau). Don samfuran yau, ana ba da shawarar Hi-Swab DNA Kit da TIANamp Bacteria DNA Kit.

Tambaya: Yadda ake zaɓar kayan don hakar gDNA daga samfuran fungi?

Kit ɗin Shuka na Tsaro na DNA ko Tsarin Tsirrai na DNAquick ana ba da shawarar don haɓakar ƙwayoyin halittar fungal. Don haɓakar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar cuta, ana ba da shawarar Kit ɗin DNA na Yisti TIANamp (lyticase yakamata a shirya shi da kansa).

Kit ɗin Haɗin Dna na Cell Free