TGuide Virus DNA/RNA Kit

Don cire kwayar cutar DNA/RNA daga magani, plasma, ruwan jikin da babu sel ko maganin rigakafin ƙwayar cuta.

TGuide Virus DNA/RNA Kit an tsara shi musamman don cire nucleic acid daga ƙwayar cuta ta amfani da TGuide Series Automated Nucleic Acid Extractor. An kula da abubuwan amfani da filastik da aka yi amfani da su a cikin kayan don cire DNase/RNase, kuma kowane samfurin yana gudanar da kansa. Tsarin zai iya guje wa yuwuwar dama daban-daban na gurɓatawa tsakanin samfurori. Kit ɗin zai iya fitar da ƙwayar cuta ta DNA ko RNA daga samfuran 200 μl /400 μl lokaci guda. Yana da tattalin arziki da dacewa don amfani.

Cat. A'a	Girman shiryawa
Saukewa: OSR-M202	48 prep

Aika mana imel

Bayanin samfur

Misalin Gwaji

Tambayoyi

Alamar samfur

Aikace -aikace

Cikakken nucleic acid ya dace da babban PCR, RTPCR, PCR mai yawa, RT-PCR mai yawa da sauran gwaje-gwaje. An tabbatar da kit ɗin ta hanyar binciken ƙasa na HBV, HCV, HIV da ƙwayoyin mura.

Siffofin

■ Sauƙaƙe da hakar mai sauri: samfuran kayan haɗi na TGuide sun dogara ne akan ƙa'idar tsarkakewa ta nucleic acid ta beads magnetic, kuma ana iya kammala aiwatar da hakar nucleic acid a cikin mintoci 57.
■ Babu gurɓatawa: An riga an cika kwandunan reagent masu zaman kansu don hana kamuwa da cuta.
Yield Yawan amfanin ƙasa: Kit ɗin ya ƙunshi RNA Carrier mai inganci don haɓaka ingancin kamawar sinadarin nucleic.
■ Babu phenol da haɓakar chloroform: Babu buƙatar ƙwayoyin ƙwayoyin cuta masu cutarwa ga jikin ɗan adam kamar phenol da chloroform.
Sample Samfurin samfurin mai ƙima na farko: Za a iya fitar da samfuran 200 μl/400 μl kai tsaye tare da abubuwan da suka dace.

Duk samfuran ana iya keɓance su don ODM/OEM. Don cikakkun bayanai,don Allah danna Sabis na Musamman (ODM/OEM)

Na baya: Kit ɗin Fitar da DNA na TGuide Plasma (1.2ml)

Na gaba: TGuide Plant Genomic DNA Kit

Fluorescence ƙimar PCR na HBV

Hoto 1: Tsarkake kwayar cutar positivel mai kyau containingl mai dauke da HBV mai yawa daban -daban ta amfani da Kit ɗin TGuide Virus DNA/RNA. An narkar da kwayar cutar ta HBV ta DNA a cikin buhun allurar μl 60.
Binciken PCR na gida na HCV

Hoto na 2: Cire samfuran magunguna waɗanda ke ɗauke da adadi daban -daban na ƙwayar cutar ta HCV ta TGuide Virus DNA/RNA Kit, kuma gano sakamakon ta PCR mai ƙwanƙwasa.
1: 5 × 10⁰ HCV Magani 2: 5 × 10¹ HCV Magani
3: 5 × 10² HCV Magani 4: 5 × 10³ HCV Magani
5: 5 × 10⁴ HCV Magani 6: 5 × 10⁵ HCV Magani
P: Kyakkyawan iko N: Ikon sarrafawa
M: 100 bp tsani na DNA

Tambaya: Ƙananan ko babu DNA a cikin mafi girma.

A-1 Ƙananan ƙwayoyin sel ko ƙwayoyin cuta a cikin samfurin farawa-Inganta yawan ƙwayoyin sel ko ƙwayoyin cuta.

A. An ba da shawarar a gauraya sosai ta hanyar bugun jini don sau 1-2. - Rashin isasshen ƙwayar sel wanda ke haifar da raguwar aiki na proteinase K. - Rashin isasshen ƙwayar sel ko lalacewar furotin saboda ƙarancin lokacin wanka mai ɗumi. Ana ba da shawarar a yanke nama a ƙananan ƙananan kuma ƙara lokacin wanka don cire duk abin da ke cikin lysate.

A-3 Rashin isasshen talla na DNA. —Ba ethanol ko ƙaramin kashi maimakon 100% ethanol da aka ƙara kafin a canza lysate zuwa ginshiƙi.

A. -Daidaita pH tsakanin 8.0-8.3.

Tambaya: DNA ba ya yin aiki da kyau a cikin gwaje -gwajen halayen enzymatic na ƙasa.

Ethanol mai raguwa a cikin mafi girma.

- Akwai rakodin wanki na PW a cikin sama. Ana iya cire ethanol ta hanyar karkatar da juzu'in juzu'i na mintuna 3-5, sannan a sanya shi a ɗaki mai ɗumi ko 50 ℃ incubator na 1-2 min.

Tambaya: Rushewar DNA

A-1 Samfurin ba sabo bane. - Cire samfurin DNA mai kyau azaman sarrafawa don sanin ko DNA a cikin samfurin ya ƙasƙantar.

A-2 Aiki kafin magani. -Yana haifar da yawan iskar nitrogen mai narkewa, dawo da danshi, ko yawan samfurin.

Tambaya: Yadda ake yin prereatment don hakar gDNA?

Dole ne shirye -shiryen shiryawa ya bambanta don samfurori daban -daban. Don samfuran shuka, tabbatar da niƙa sosai a cikin nitrogen mai ruwa. Don samfuran dabbobi, homogenate ko niƙa sosai a cikin nitrogen mai ruwa. Don samfuran da ke da bangon sel waɗanda ke da wuyar fashewa, kamar ƙwayoyin G+ da yisti, ana ba da shawarar yin amfani da lysozyme, lyticase ko hanyoyin inji don fasa katangar tantanin halitta.

Tambaya: Menene banbanci tsakanin kayan hakar gDNA guda uku 4992201/4992202, 4992724/4992725, 4992709/4992710?

4992201/4992202 Plant Genomic DNA Kit ya ɗauki hanyar tushen shafi wanda ke buƙatar chloroform don hakar. Ya dace musamman ga samfuran tsire -tsire iri -iri, kazalika da busasshen foda. Kit ɗin Tsarin Hi-DNAsecure shima tushen tushen shafi ne, amma ba tare da buƙatar hakar phenol/chloroform ba, yana mai da shi lafiya kuma ba mai guba ba. Ya dace da tsire -tsire masu yawan polysaccharides da abubuwan polyphenol. 4992709/4992710 DNAquick Plant System yana ɗaukar hanyar tushen ruwa. Ba'a buƙatar hakar Phenol/chloroform shima. Tsarin tsarkakewa yana da sauƙi da sauri ba tare da iyakance ga adadin farkon samfurin ba, don haka masu amfani za su iya daidaita adadin a hankali gwargwadon buƙatun gwaji. Ana iya samun babban gutsuttsuran gDNA tare da yawan amfanin ƙasa.

Menene ƙididdigar ƙimar gDNA daga samfurin jini na 1 ml ta Kit ɗin DNA na Jini na TIANamp?

An samo kwayar halittar DNA daga juzu'i daban -daban na samfuran jinin ɗan adam ta Kit ɗin DNA na Jini. Sakamakon shine kamar haka. An jera sakamakon a matsayin tunani kawai, ainihin sakamakon hakar ya dogara da yanayin samfura.

Tambaya: Za a iya amfani da 4992207/4992208 da 4992722/4992723 don fitar da ɗanyen jini na DNA?

Ana iya aiwatar da haɓakar DNA na ɗigon jini ta amfani da reagents da aka bayar a cikin waɗannan kayan guda biyu ta hanyar sauƙaƙe yarjejeniya zuwa takamaiman umarni don hakar DNA na ɗigon jini. Za'a iya bayar da kwafin taushi na ƙa'idar haɓakar DNA akan buƙata.

Tambaya: Lokacin neman TIANamp Genomic DNA Kit, yadda za a fasa sabbin kyallen a cikin dakatarwar sel?

Dakatar da sabon samfurin tare da 1 ml PBS, saline na al'ada ko buhun TE. Cikakken homogenize samfurin ta homogenizer kuma tattara precipitate zuwa kasan bututu ta centrifuging. Jefar da allahntaka, kuma sake dawo da hazo tare da 200 μl buffer GA. Ana iya yin tsarkakewar DNA na gaba bisa ga umarnin.

Tambaya: Yadda za a zaɓi samfurin don haɓakar DNA daga plasma, magani da samfuran ruwa?

Don tsabtace gDNA a cikin plasma, magani da samfuran ruwa, ana bada shawarar TIANamp Micro DNA Kit. Don tsabtace ƙwayar gDNA daga samfuran serum/plasma, ana bada shawarar TIANamp Virus DNA/RNA Kit. Don tsabtace gDNA na kwayan cuta daga samfuran jini da samfuran plasma, ana bada shawarar TIANamp Bacteria DNA Kit (yakamata a haɗa lysozyme don ƙwayar cuta mai kyau). Don samfuran yau, ana ba da shawarar Hi-Swab DNA Kit da TIANamp Bacteria DNA Kit.

Tambaya: Yadda ake zaɓar kayan don hakar gDNA daga samfuran fungi?

Kit ɗin Shuka na Tsaro na DNA ko Tsarin Tsirrai na DNAquick ana ba da shawarar don haɓakar ƙwayoyin halittar fungal. Don haɓakar ƙwayar ƙwayar ƙwayar ƙwayar ƙwayar cuta, ana ba da shawarar Kit ɗin DNA na Yisti TIANamp (lyticase yakamata a shirya shi da kansa).

Kit ɗin Haɗin Dna na Cell Free