RNAprep Pure Hi-Blood Kit

Don tsarkakewa na RNA mai inganci da tsayayye daga jini.

RNAprep Pure Hi-Blood Kit yana aiki da kyau yana fitar da jimlar RNA daga sabbin jini da jini tare da magungunan kashe ƙwayoyin cuta masu yawa daga nau'ikan daban-daban. Abubuwan matrix na silicon da aka yi amfani da su a cikin tallan talla shine sabon sabon abu wanda TIANGEN ya haɓaka, wanda ya dace kuma musamman yana tallata RNA, kuma yana cire sunadarai marasa ƙazanta. Ana iya amfani da RNA da aka fitar a cikin gwaje-gwaje daban-daban na ƙasa kamar RT-PCR, RT-qPCR, bincike na guntu, babban jerin abubuwan da aka tsara, Northern Blot, Dot Blot, gwajin PolyA, fassarar in vitro, nazarin kariya na RNase, cloning kwayoyin halitta, da sauransu.

Cat. A'a	Girman shiryawa
4992903	50 prep

Aika mana imel

Bayanin samfur

Misalin Gwaji

Tambayoyi

Alamar samfur

Siffofin

Itable Ya dace da sabon jini gaba ɗaya na nau'ikan daban -daban, mai sauƙin aiki.
■ Sanye take da Rumun Ramin Rataye na RNase-Free CS don kawar da ƙazanta.
Buff Abubuwan da aka tsara musamman na iya tabbatar da haɓakar haɓakar haɓakar RNA don gwaje -gwaje iri -iri.
■ Amintaccen aiki mai aminci, babu buƙatar hakar phenol/chloroform.

Aikace -aikace

RT-PCR, Northern Blot, RT-qPCR, bincike na guntu, jerin abubuwan da aka tsara sosai, gwajin PolyA, nazarin kariyar RNase, fassarar in vitro, da sauransu.

Duk samfuran ana iya keɓance su don ODM/OEM. Don cikakkun bayanai,don Allah danna Sabis na Musamman (ODM/OEM)

Na baya: RNAprep Pure Plant Plus Kit

Na gaba: RNA Easy Fast Tissue/Cell Kit

	Hoto 1. RNA da aka tsarkake daga 100 μl sabon jinin bera a cikin magungunan kashe kashe daban-daban ta amfani da RNAprep Pure Hi-Blood Kit. An ɗora 4-6 ofl na 50 atesl eluates ta kowane layi. M: TIANGEN DNA Alamar III.
	Hoto 2. RNA ta tsarkake daga 100 μl sabon jinin linzamin kwamfuta ta amfani da RNAprep Pure Hi-Blood Kit. An ɗora 4-6 ofl na 50 atesl eluates ta kowane layi. M: TIANGEN DNA Alamar III.

Tambaya: Toshewar shafi

A-1 Cell lysis ko homogenization bai isa ba

---- Rage amfani da samfur, ƙara yawan adadin lysis, ƙara homogenization da lokacin lysis.

A-2 Samfurin adadin ya yi yawa

---- Rage adadin samfurin da aka yi amfani da shi ko ƙara yawan adadin kuzarin lysis.

Tambaya: Ƙananan amfanin RNA

A-1 Rashin isasshen lysis na sel ko homogenization

---- Rage amfani da samfur, ƙara yawan adadin lysis, ƙara homogenization da lokacin lysis.

A-2 Samfurin adadin ya yi yawa

---- Don Allah koma zuwa matsakaicin ƙarfin sarrafawa.

A-3 RNA ba a fitar da shi gaba ɗaya daga shafi

---- Bayan ƙara ruwa RNase-Free, bar shi na 'yan mintoci kaɗan kafin a datse.

A-4 Ethanol a cikin mafi girma

---- Bayan rinsing, sake maimaita centrifuge kuma cire buhun wanki gwargwadon iko.

Ba a cire matsakaicin al'adar C-5 gaba ɗaya

---- Lokacin tattara sel, don Allah a tabbata an cire matsakaitan al'adu gwargwadon iko.

A-6 Kwayoyin da aka adana a cikin RNAstore ba su da tsattsauran ra'ayi

---- Yawan RNAstore ya fi matsakaicin matsakaicin al'adar salula; don haka yakamata a kara karfin centrifugal. An ba da shawarar yin centrifuge a 3000x g.

A-7 Ƙananan abun cikin RNA da yalwa a cikin samfurin

---- Yi amfani da samfuri mai kyau don sanin idan ƙarancin samfurin ya haifar da samfurin.

Tambaya: ƙasƙantar da RNA

A-1 Kayan ba sabo bane

---- Yakamata a adana sabbin kyallen takarda a cikin sinadarin nitrogen nan da nan ko kuma a saka su cikin reagent na RNAstore don tabbatar da tasirin hakar.

A-2 Samfurin adadin ya yi yawa

---- Rage adadin samfurin.

A-3 RNase gurbatawan

---- Ko da yake buffen da aka bayar a cikin kit ɗin bai ƙunshi RNase ba, yana da sauƙi a gurɓata RNase yayin aikin hakar kuma ya kamata a kula da shi sosai.

A-4 gurɓataccen Electrophoresis

---- Sauya mazubin electrophoresis kuma tabbatar da cewa abubuwan amfani da Load Buffer ba su da gurɓataccen RNase.

A-5 Ana yin lodin yawa don electrophoresis

---- Rage adadin lodin samfurin, lodin kowace rijiya kada ya wuce 2 μg.

Tambaya: Gurɓacewar DNA

A-1 Samfurin adadin ya yi yawa

---- Rage adadin samfurin.

A-2 Wasu samfurori suna da babban abun ciki na DNA kuma ana iya bi da su tare da DNase.

---- Yi maganin DNase na RNase-Free zuwa maganin RNA da aka samu, kuma ana iya amfani da RNA kai tsaye don gwaje-gwajen da suka biyo baya bayan magani, ko kuma za'a iya ƙara tsarkakewa ta kayan aikin tsarkakewa na RNA.

Tambaya: Yadda za a cire RNase daga kayan gwaji da gilashin gilashi?

Don gilashin gilashi, gasa a 150 ° C na tsawon awanni 4. Don kwantena na filastik, nutse cikin 0.5 M NaOH na mintuna 10, sannan a rinshe shi da ruwa mara RNase sannan a yi taɓo don cire RNase gaba ɗaya. Reagents ko mafita da aka yi amfani da su a cikin gwaji, musamman ruwa, dole ne su kasance marasa RNase. Yi amfani da ruwa mara RNase don duk shirye-shiryen reagent (ƙara ruwa zuwa kwalban gilashi mai tsabta, ƙara DEPC zuwa taro na ƙarshe na 0.1% (V/V), girgiza dare da autoclave).

Rubuta saƙonka a nan ka aika mana