Kit ɗin RNAclean

A-1 Cell lysis ko homogenization bai isa ba

---- Rage amfani da samfur, ƙara yawan adadin lysis, ƙara homogenization da lokacin lysis.

A-2 Samfurin adadin ya yi yawa

---- Rage adadin samfurin da aka yi amfani da shi ko ƙara yawan adadin kuzarin lysis.

A-1 Rashin isasshen lysis na sel ko homogenization

---- Rage amfani da samfur, ƙara yawan adadin lysis, ƙara homogenization da lokacin lysis.

A-2 Samfurin adadin ya yi yawa

---- Don Allah koma zuwa matsakaicin ƙarfin sarrafawa.

A-3 RNA ba a fitar da shi gaba ɗaya daga shafi

---- Bayan ƙara ruwa RNase-Free, bar shi na 'yan mintoci kaɗan kafin a datse.

A-4 Ethanol a cikin mafi girma

---- Bayan rinsing, sake maimaita centrifuge kuma cire buhun wanki gwargwadon iko.

Ba a cire matsakaicin al'adar C-5 gaba ɗaya

---- Lokacin tattara sel, don Allah a tabbata an cire matsakaitan al'adu gwargwadon iko.

A-6 Kwayoyin da aka adana a cikin RNAstore ba su da tsattsauran ra'ayi

---- Yawan RNAstore ya fi matsakaicin matsakaicin al'adar salula; don haka yakamata a kara karfin centrifugal. An ba da shawarar yin centrifuge a 3000x g.

A-7 Ƙananan abun cikin RNA da yalwa a cikin samfurin

---- Yi amfani da samfuri mai kyau don sanin idan ƙarancin samfurin ya haifar da samfurin.

A-1 Kayan ba sabo bane

---- Yakamata a adana sabbin kyallen takarda a cikin sinadarin nitrogen nan da nan ko kuma a saka su cikin reagent na RNAstore don tabbatar da tasirin hakar.

A-2 Samfurin adadin ya yi yawa

---- Rage adadin samfurin.

A-3 RNase gurbatawan

---- Ko da yake buffen da aka bayar a cikin kit ɗin bai ƙunshi RNase ba, yana da sauƙi a gurɓata RNase yayin aikin hakar kuma ya kamata a kula da shi sosai.

A-4 gurɓataccen Electrophoresis

---- Sauya mazubin electrophoresis kuma tabbatar da cewa abubuwan amfani da Load Buffer ba su da gurɓataccen RNase.

A-5 Ana yin lodin yawa don electrophoresis

---- Rage adadin lodin samfurin, lodin kowace rijiya kada ya wuce 2 μg.

A-1 Samfurin adadin ya yi yawa

---- Rage adadin samfurin.

A-2 Wasu samfurori suna da babban abun ciki na DNA kuma ana iya bi da su tare da DNase.

---- Yi maganin DNase na RNase-Free zuwa maganin RNA da aka samu, kuma ana iya amfani da RNA kai tsaye don gwaje-gwajen da suka biyo baya bayan magani, ko kuma za'a iya ƙara tsarkakewa ta kayan aikin tsarkakewa na RNA.

Don gilashin gilashi, gasa a 150 ° C na tsawon awanni 4. Don kwantena na filastik, nutse cikin 0.5 M NaOH na mintuna 10, sannan a rinshe shi da ruwa mara RNase sannan a yi taɓo don cire RNase gaba ɗaya. Reagents ko mafita da aka yi amfani da su a cikin gwaji, musamman ruwa, dole ne su kasance marasa RNase. Yi amfani da ruwa mara RNase don duk shirye-shiryen reagent (ƙara ruwa zuwa kwalban gilashi mai tsabta, ƙara DEPC zuwa taro na ƙarshe na 0.1% (V/V), girgiza dare da autoclave).