Kit ɗin Nazarin HRM (EvaGreen)

Siffofin

Resolution Babban ƙuduri: Dauki EvaGreen cikakken dyes, wanda ke da babban ƙudurin narkewa a cikin cikakken yanayin kuma yana iya rarrabe bambancin tushe ɗaya.
■ Babban keɓaɓɓen bayani: Yi amfani da antibody modified hotstart DNA polymerase don rage haɓakawa ba takamaimai da haɓaka keɓancewa ba.
Stability Babban kwanciyar hankali: A hankali inganta tsarin Buffer yana haɓaka kwanciyar hankali na narkewa mai lankwasa da haɓaka amincin sakamakon.
Corre Gyara ROX: An haɗa fenti na ROX daban, wanda yafi sassauƙa don amfani da samun ingantattun sakamako.

Musammantawa

Nau'in: Antibody ya canza Taq DNA polymerase, EvaGreen.
Aikace -aikace: Sanannen buga SNP; Binciken SNP da ba a sani ba; Binciken kwayoyin halittar da ba a sani ba; Methylation PCR bincike.

Duk samfuran ana iya keɓance su don ODM/OEM. Don cikakkun bayanai,don Allah danna Sabis na Musamman (ODM/OEM)

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An fitar da DNA na kwayar halitta daga μl na samfurin jinin ɗan adam ta amfani da Kit ɗin DNA na Jinin TIANamp. An ɗora 50 ng DNA don ainihin gano PCR. Dangane da ɗakin karatu na NCBI SNP, an zaɓi sanannen SNP na FEN1 gene (RS 174538) kuma an gano shi ta amfani da Roche LightCycle480. Sakamakon haka kamar haka:

Sakamakon ya nuna cewa Kit ɗin Tattaunawar HRM shine ingantacciyar hanyar nazarin yanki na SNP tare da maimaita maimaita narkewar lanƙwasa iri ɗaya, bayyananniyar ƙuduri na nau'ikan halittu daban -daban kuma babu yanke hukunci.

Bayanin SNP: …… CGGAAGAACACGTCG [A/G] CAGGAGCAGGCGCCT ……

Farko: Don guntun bp na 108 (FEN1_F: CCTCAACGCTCTCACCATTTTG; FEN1_R: GGCACTTCCTTTCCGGTTGTG)