FastKing Mataki Daya Kit RT-qPCR

-Aya-mataki Real-Time RT-PCR tare da SYBR-Green.

FastKing Stepaya Mataki RT-qPCR Kit an tsara shi don Lokaci-Lokaci Stepaya Mataki RT-qPCR ta amfani da SYBR Green I. Za'a iya amfani da kit ɗin a cikin lokaci-lokaci mataki ɗaya RT-qPCR na makasudin RNA, tare da jujjuyawar juyawa da PCR suna faruwa a jere. a cikin bututu guda. Saboda haka aikin yana da sauƙi, kuma yana rage haɗarin kamuwa da cuta.

Haɗin 25 × RT-PCR Enzyme Mix a cikin wannan kit ɗin shine jigon littafin TIANGEN na sabon juyi transcriptase (FastKing RTase), sabon antibody modified hotstart Taq DNA polymerase da RNase Inhibitor. FastKing RTase shine sabon nau'in nau'in juzu'in juzu'in juzu'in juzu'i. Tare da musamman hydrophobic motif, wanda ke da ƙarfi RNA dangantaka da kwanciyar hankali na zafi. FastKing RTase ya inganta ingantaccen juzu'in juzu'in juyawa da ikon fadada samfurin RNA tare da hadaddun tsarin sakandare. Sabuwar nau'in hotstartTaq Ana amfani da polymerase na DNA tare da kyakkyawan aiki a cikin tsarin PCR, yana mai da martani na PCR bayan jujjuyawar juzu'i yana da ingantaccen haɓakawa da ƙayyadaddun bayanai. Bugu da kari, 2 × FastKing RT-qPCR Buffer (SYBR Green) a cikin wannan samfurin sabon tsarin amsawa ne wanda aka inganta musamman don manyan enzymes biyu na sama, gami da abubuwan da ake buƙata na ionic, dNTPs, SYBR Green rye, mai daidaita mataki ɗaya da haɓakawa, wanda iya tabbatar da cewa FastKing RTase da sabon hotstartTaq DNA polymerase na iya taka rawar da ta fi tasiri a cikin duk tsarin amsa mataki ɗaya.

Wannan kit ɗin yana ba da madaidaicin madaidaiciyar madaidaiciya a cikin yanki mai yawa. Yana iya daidai da adadi da yawa don gano iri -iri iri -iri masu ƙima da ƙanƙantar da niyya tare da kyakkyawan maimaitawa da babban dogaro.