Kit ɗin Plasmid na EndoFree Maxi V2

Tsarkake matakin plasmid DNA kyauta na endotoxin kyauta ga sel masu mahimmanci.

Kit ɗin EndoFree Maxi Plasmid V2 yayi amfani da dabarun tallan membrane na silica na musamman don daɗaɗa da musamman ɗaurin DNA na plasmid. Buffer ER na musamman da Filtration CS1 na iya kawar da ƙazanta kamar endotoxin da furotin. Gabaɗayan tsarin hakar yana ɗaukar awa 1 kawai.
Adadin matsakaicin al'adar ƙwayoyin cuta: Don babban kwafin kwaro, ana ba da shawarar matsakaicin ƙwayar ƙwayar ƙwayar cuta ta 100 ml, wanda zai iya haifar da plasmid 500-1500 μg. Don plasmid mai ƙarancin kwafi, ana ba da shawarar 200 ml na matsakaitan ƙwayoyin cuta don cimma yawan amfanin ƙasa kusan 50-300 μg.

Cat. A'a Girman shiryawa
4992438 10 prep

Bayanin samfur

Gudun aiki

Misalin Gwaji

Alamar samfur

Siffofin

Urity Babban tsarki: An karɓi fasahar hazo na musamman na endotoxin don cire endotoxin musamman.
■ Mai sauƙin aiki: Ana amfani da fasahar shafi na tallan don tallata DNA na plasmid, wanda ke sauƙaƙa aikin.
Trans Ƙaƙƙarfan ƙazantawa mai dacewa: Ya dace da jujjuyawar yawancin layukan salula gami da ƙwayoyin endotoxin.
Range Aikace -aikace masu yawa: Za a iya amfani da plasmid ɗin da aka tsarkake a cikin jujjuyawar ƙwayoyin dabbobi da tsire -tsire har ma da gwaje -gwajen nazarin halittu.

Aikace -aikace

DNA na plasmid da aka fitar ta amfani da wannan kit ɗin ana iya amfani dashi don ayyuka daban -daban na yau da kullun, gami da ƙuntata narkewar enzyme, PCR, jere, haɗawa, canzawa da canza sel daban -daban.

Duk samfuran ana iya keɓance su don ODM/OEM. Don cikakkun bayanai,don Allah danna Sabis na Musamman (ODM/OEM)


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    Workflow

    Experimental Example 图片 2 Buƙatar ER na musamman mai cire kayan endotoxin na iya cire ragowar endotoxin a cikin tsarin amsawa, kuma ana iya samun plasmid mai tsafta. Ragowar endotoxin plasmid shine ≤ 0.1 EU/ml.
    Experimental Example An tsarkake plasmid ɗin ta amfani da EndoFree Maxi Plasmid Kit V2 da samfuran iri ɗaya daga Mai Bayarwa 1 da Mai Bayarwa na 2 an ƙera su da girman ƙimar allurar. An ɗora 1 plasl plasmid ta kowace layi don kimanta mahimmancin plasmid. 100 ng na plasmid wanda ƙimar spectrophotometer ta ƙaddara shi an ɗora shi a cikin gel ɗin ɗaya don sanin ko ƙimar taro ya yi ƙarya.
    Kammalawa: Sakamakon zaɓin electrophoresis yana nuna ainihin maida hankali na plasmid da EndoFree Maxi Plasmid kit V2 ya fitar, ko babban kwafi ne ko ƙaramin kwafi, yana da girma fiye da wanda masu samar da kayayyaki 1 da 2 suka fitar.
    Rubuta saƙonka a nan ka aika mana